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PRINCIPLE
To detect and monitor cultures for mold and mildew or potential infectious agents in a tested facility.
REAGENTS/SUPPLIES
Sterile collection swabs
METHOD WITHOUT USE OF AIR PURIFIER
- Select areas of facilities to be tested. Example: vents, dust from door ledge, drain pans, or growth on sur-face, etc.
- Use sterile swab to collect sample by swabbing area(s) in a 1 square inch fashion.
- Re-cap sterile swab and label appropriately (date, location, etc.)
- Package swab and send to laboratory via standard mail.
METHOD WITH USE OF AIR PURIFIER
- Select area of facility to be tested. Example: vents, dust from door ledge, drain pans, or growth on surface, etc.
- Use FIRST sterile swab to collect sample by swabbing selected area in a 1 square inch fashion.
- Re-cap sterile swab and label appropriately (date, location, etc.)
- Turn on air purifier and wait five calendar days.
- Use SECOND sterile swab to collect sample by swabbing same area in a 1 square inch in exactly the same manner as the first swab.
- Package swabs and send to laboratory via standard mail.
INTERPRETATION
- Swab will be plated at the laboratory on supportive agar and incubated at 25-30 degrees C.
- Cultures will be observed for growth over a 5-10 day period.
- Colonies grown will be identified and a measure of growth will be noted.
POTENTIAL ORGANISMS
Aspergillus sp., Stachybotrys sp., Cladosporium sp., Curvularia sp., Penicillium sp., Rhizopus sp., Mucor sp., Alternaria sp., Fusarium sp., etc. and yeast, if present.
View a sample
report.
Author: Glenn Moore, BS, MT, Microbiology Supervisor
Florida License #SU30654
727.804.4206
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